FIG. 6.

Effect of neuron-specific PTP1B deficiency on the AMPK pathway and UCP1 protein expression in BAT. (A) α1 and α2 AMPK activities. (B to D) Immunoblotting of α AMPK phosphorylation, α1 and α2 AMPK, UCP1, and β-actin protein levels. Data in panels B and C are normalized to β-actin. All experiments were carried out using 2-month-old male flox/flox (fl/fl) control and neuronal PTP1B^−/− mice on a chow diet. Data are expressed as means ± SEM; n = 6 to 8 for each genotype. *, P < 0.05 compared to results for fl/fl mice.