FIG. 1.

Coimmunoprecipitation of PFAAP5, Gfi1, and neutrophil elastase. NIH 3T3 cells were transfected with Gfi1, neutrophil elastase (NE), and PFAAP5 expression vectors (or a β-galactosidase-expressing control). In each set, the Myc epitope tag was present in one or none (fourth lane from left) of the expression vectors. Input lysates before (left blot) or after (right blot) immunoprecipitation with anti-Myc (α-Myc) were detected by Western blotting with anti-Gfi1 (top row), anti-neutrophil elastase carboxyl-terminal propeptide (middle row), and anti-PFAAP5 (bottom row). Transfection with a β-galactosidase-expressing control vector was included to demonstrate antibody specificity in input Western blots. (For the PFAAP5 input Western blot, the lanes are cropped from a single exposure of a continuous membrane, as indicated with a vertical line separating the rightmost lane.)