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Quantification of procaspase-8 processing. SKW6.4 cells were stimulated for different times with 1 μg/ml of anti-APO-1 MAb (A) or with different amounts of anti-APO-1 MAb for 1 h (B). Procaspase-8 processing at the CD95 DISC was analyzed by Western blotting using the anti-caspase-8 MAb C15. The relative distribution of procaspase-8 cleavage products detected by the anti-caspase-8 MAb C15 was quantified (*, unspecific band).
