FIG. 6.

p30 can be processed to p10 and p18 by active caspase-8 and -9. (A) Recombinant caspase-3, -8, or -9 was added to in vitro-translated ³⁵S-labeled HAp30 and the uncleavable mutant D374/384A of HAp30 (HAp30mut). Processing was visualized by autoradiography (*, unspecific band). (B) 293T cells were transfected with HAp30 and HAp30 mut. Recombinant caspase-3, -8, or -9 or stimulated lysate (lys) of SKW6.4 cells was added to HA immunoprecipitates, and processing of p30 was analyzed by Western blotting using the anti-caspase-8 MAbs C15 and C5 and the anti-HA MAb 12CA (*, unspecific band).