FIG. 3.

Mutations in TORC2 confer sensitivity to DNA-damaging conditions independently of Cds1 or Chk1. (A and B) Tor1 functions independently of Chk1 or Cds1. Serial dilutions of mutant cells were plated with or without the indicated amounts of HU or MMS. (C) Tor1 is not required for phosphorylation of Chk1. Western blot analysis of HA-tagged Chk1. Wild-type or Δtor1 cells containing HA-tagged Chk1 were grown to log phase. Protein was extracted from untreated cells or treated with 0.2% MMS for the indicated times (minutes).