Figure 1.

Confirmation of Knockout of TR4 Gene in TR4^−/− Female Mice

A, PCR analysis of mouse genomic DNA. The wild-type (+/+) and knockout (−/−) give 455- and 760-bp PCR products, respectively. B, Expression of TR4 mRNA in ovarian tissue from TR4^+/+ and TR4^−/− female mice by Q-PCR (n = 4 mice per group). C–F, Immunostaining of TR4 protein in ovarian sections from TR4^+/+ and TR4^−/− mice: C and D, TR4^−/− ovary shows no TR4 staining in granulosa cell and oocyte; E and F, in TR4^+/+ ovary, TR4 staining was found in oocytes and granulosa cells of primary, preantral, and antral follicles and was found in lutea cells of CL.