Figure 3.

The Effect of NFATc1 on Expression of Various Genes during RANKL-Induced Osteoclast Fusion

A–C, To generate preosteoclasts, BMMs were cultured for 48 h in the presence of M-CSF and RANKL. Preosteoclasts were incubated for an additional 24 h with M-CSF, in the absence (Vehicle) or presence of RANKL with the various indicated concentrations of CsA. A, Cultured cells were fixed and stained for TRAP. B, TRAP(+) MNCs having more than three nuclei were counted as osteoclasts. Data represent means ± sd of triplicate samples. C, RT-PCR was performed to detect expression of the indicated genes.