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. 2009 Jan 15;23(4):454–465. doi: 10.1210/me.2008-0267

Figure 1.

Figure 1

Pg decreases the E2-induced cell proliferation in breast cancer cells. A, Immunoblot analysis of PR. MCF-7 cells were treated with vehicle (−) or 10 nm E2 at different time as indicated; GAPDH was used as loading control. Results are representative of three independent experiments. B, MCF-7, ZR75, and T47D cells were treated with vehicle (−) or 10 nm E2 and/or increasing amount of Pg (1 nm, 10 nm, 100 nm) in medium containing 5% charcoal-stripped fetal bovine serum (medium was refreshed and treatments were renewed every 2 d) and counted on d 6. C, MCF-7, ZR75, and T47D cells cultured in the experimental conditions described in panel B were also treated with vehicle (−) or 10 nm E2 and/or 10 nm Pg in combination with 1 μm RU or 1 μm OHFl and counted on d 6. D, MCF-7 cells were transfected with nonspecific siRNA or targeted against PR-B and cultured in the experimental conditions described in panel B. Columns indicate mean of three independent experiments done in triplicate; bars represent sd; *, P < 0.001 compared with vehicle; **, P < 0.001 compared with E2; ^, P < 0.001 compared with E2+Pg; •, P < 0.001 vs. cells treated with E2+Pg transfected with nonspecific siRNA.