Figure 5.

Estradiol and Progesterone Production by Undifferentiated Granulosa Cells Stimulated with FSH or cAMP Analogs

Undifferentiated rat granulosa cells were plated overnight in 48-well plates of M199 containing 10% CS. The next morning, media and unattached cells were removed and replaced with M199. After 24 h, medium was removed and replaced with fresh medium containing 30 ng/ml testosterone with or without FSH at 100 ng/ml and 6-Bnz-cAMP or 6-MB-cAMP at concentrations of 0.5, 1, and 2 mm. Forty-eight hours later, medium was collected and analyzed for estradiol (top) and progesterone (bottom) content by RIA. Results show mean ± 1 sem of four groups of granulosa cells. Significant differences were considered when P < 0.05 and are indicated by different letters in the figure.