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. 2009 May 14;83(4):768–777. doi: 10.1093/cvr/cvp150

Figure 6.

Figure 6

Effects of cHSP60 on eNOS expression, superoxide anion production, and vasomotor functions in porcine coronary arteries. Porcine right coronary artery rings were cultured with cHSP60 (0.2, 2, or 10 µg/mL), with or without antioxidant SeMet (20 µM) for 24 h. (A) The eNOS mRNA levels were determined by real-time PCR. *P < 0.05, **P < 0.01, n = 4, t-test. (B) The superoxide anion (O−2) levels were determined by lucigenin-enhanced chemiluminescence assay. The data were normalized with area (mm2) of the ring and are expressed as relative light units (RLU/s/mm2). **P < 0.05, n = 6, t-test. (C) Vasomotor function was determined by myograph analysis for cHSP60 (0.2, 2, or 10 µg/mL) treatment group. After cHSP60 treatment, porcine right coronary artery rings were pre-contracted with thromboxane A2 analogue U46619 (3 × 10−8 M) and then subjected to endothelium-dependent relaxation by adding a series of concentrations of bradykinin (10−9–10−5 M). *P < 0.05, n = 10, ANOVA. (D) Vasomotor functions determined by myograph analysis for HI-cHSP60, SeMet, and SeMet plus cHSP60 treatment groups. The endothelium-dependent relaxation in response to a series of concentrations of bradykinin in each treatment group did not show any change compared with untreated controls.