Figure 4.

Effects of CF-conditioned media on the presence and phosphorylation of Cx43 in cardiac monolayers. (A) Representative stainings of total Cx43 in control (A1) and conditioned (A2) monolayers. (A3) Total Cx43 area per nucleus quantified from n = 5 monolayers per group. (B) Representative stainings of UnPO₄ Cx43 in control (B1) and conditioned (B2) monolayers. Inset, positive control in hypoxic cardiomyocytes. Arrows point to positive staining (red). (B3) UnPO₄ Cx-43 area per nucleus quantified from n = 5 monolayers per group. (C) Merged images of control (C1) and conditioned (C2) monolayers. (C3) The ratio of UnPO₄-Cx43/total Cx43. (D) Time course of FRAP in control (D1) and conditioned (D2) cells averaged from n = 4 cells per group. (D3) The resulting transfer constants (k). Asterisks denote significantly different results from control.