Figure 5.
Effects of CF-conditioned media on cardiac gene expression and single cell electrophysiology. (A) Gene expression in conditioned relative to control cultures. Cx, connexin; SCN5A, cardiac sodium channel NaV1.5 α-subunit; KCNJ2, inwardly rectifying potassium channel (Kir2.1); MHC, myosin heavy chain; KCND3, transient outward potassium channel (Kv4.3) was downregulated to an undetectable level by conditioning. (B) Representative cardiac action potentials traces from 2 Hz paced conditioned and control monolayers. (C1–3) Action potential parameters obtained from n = 18 (control) and 39 (conditioned) cells. Asterisks denote significantly different results from control.