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Examples of mass spectra illustrating the four-level scale of heavy-atom reactivity based on the derivative peak height. (a) 10 mM buffer; (b) test case carried out in 50 mM buffer. The His-peptide derivatized by potassium tetrachloroaurate in highly reactive MES buffer at pH 6.0 (+++), moderately reactive sodium acetate buffer at pH 5.0 (++), minimal reactive sodium citrate buffer at pH 5.0 (+) and nonreactive HEPES buffer at pH 8.0 (−).
