. 2009 Jun 27;16(4):195–211. doi: 10.1093/dnares/dsp011

Table 2.

Primer sets used for analyses of HERV LTR-derived transcriptional activity

Region amplified	Primer sequence for real-time qRT–PCR (5′-3′)		Length (bp)	Hyb. temp. (°C)
ERVWE1-env^a	f	CCCCATCGTATAGGAGTCTT	207	55
ERVWE1-env^a	r	CCCCATCAGACATACCAGTT	207	55
ERVWE1[3′LTR]-U5	f	GTCCATGTTTCTTACGGCTTG	402	55
ERVWE1[3′LTR]-U5	r	GTGTTACTTGGGGGTCCTTG	402	55
HW_4[5′LTR]-U5	f	GGTTCGTGCTAATTGAGCTG	167	55
HW_4[5′LTR]-U5	r	ATGGTGGCAAGCTTCTTGTT	167	55
HW_12[solo LTR]-U5	f	CCATTGCTCCTGCACAGTTA	99	55
HW_12[solo LTR]-U5	r	TTAGAAGCAGTGGGTCATGGA	99	55
ERVFRDE1-env^a	f	GCCTGCAAATAGTCTTCTTT	114	55
ERVFRDE1-env^a	r	ATAGGGGCTATTCCCATTAG	114	55
ERV3-env^a	f	CCATGGGAAGCAAGGGAACT	141	55
ERV3-env^a	r	CTTTCCCCAGCGAGCAATAC	141	55
18S	f	TTCGGAACTGAGGCCATGAT	101	55
18S	r	TTTCGCTCTGGTCCGTCTTG	101	55
HPRT	f	GTGATGATGAACCAGGTTATGACCTTG	261	55
HPRT	r	CTACAGTCATAGGAATGGATCTATCAC	261	55

^aFrom De Parseval et al.¹¹