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. 2009 Aug 15;23(16):1929–1943. doi: 10.1101/gad.532109

Figure 1.

Figure 1.

Label-free quantitative phosphoproteomic screens. (A) TAP42 and SCH9 act in parallel downstream from TORC1. Ten-fold serial dilutions of sch9 tap42 cells complemented with indicated alleles of SCH9 and TAP42 and made prototroph with pAH149 were spotted onto the indicated media and incubated for 2–5 d at 25°C or 37°C. (Rap) Rapamycin. (B) Strategy for label-free quantitative phosphoproteomics. Triple arrows indicate steps performed in triplicate. (C) Venn diagram of phosphopeptides identified in both screens 2 and 3. Subsets of phosphopeptides found to be up-/down-regulated by rapamycin in each screen and their overlaps are shown. The overlap of phosphopeptides predicted to be down-regulated in screen 3 and up-regulated in screen 2 is not statistically significant (P = 0.25). P-values associated with the overlaps enrichment. (*) P < 10−12; (#) P < 10−24.