Table 1.

Summary of the phosphoproteomic screens

List of phosphoproteins predicted to be regulated by rapamycin in both screens and/or tested to be rapamycin-sensitive by independent assays. Proteins are sorted according to their reported functions.

^a,b(↗) Up-regulated phosphopeptides; (↘) down-regulated phosphopeptides; (—) no regulated phosphopeptides. In parentheses, regulation observed in confirmation experiments [(WB) migration shift assay in Western blot; (PS) Phosphostaining; (↗) up-regulation of phosphorylation; (↘) down-regulation of phosphorylation; (—) no change in phosphorylation].

^cPrevious reports showing that the phosphorylation of the indicated proteins is regulated by rapamycin in a similar manner as in the phosphoproteomic screens.

^dRps6a and Rps6b have identical protein sequences.

^eThe peptides identified for Hrb1 and Gbp2 mapped to regions of the proteins that are identical in sequence and therefore could not be attributed to one or the other.