Figure 2.

Evaluation of combination therapy. The objective is to provide a therapeutic that can maximize the amount of ‘free product' in the relevant biological effect compartment. A tailored mathematical model of the biological network involved in regulating the target ‘product' was developed, validated and used to explore different combinations of therapeutic intervention to select those that might be optimal. Here, it can be seen that a 1-μM dose of either anti-X or anti-Y on their own would deliver <20% or <5% free product, whereas in combination, at a tenfold lower dose of 100 nM, they delivered >40% free product. These are hypotheses generated by a validated model that can be used to focus expensive and time-consuming experimental effort more effectively.