Figure 5.

Anisomycin treatment has no effect when it does not follow a memory retrieval trial. One day after contextual fear conditioning mice either received a 3-min re-exposure to the conditioning CTX (retrieval) or were moved to the experimental procedure room and handled (no retrieval). Mice were then injected with either anisomycin or vehicle immediately and 2 h later. When tested 1 d later, mice treated with anisomycin following memory retrieval had a significant impairment, while those that did not experience a retrieval trial showed no such deficit.