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. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Cancer Lett. 2008 Feb 20;263(2):243–252. doi: 10.1016/j.canlet.2008.01.007

Figure 1.

Figure 1

Membrane extracts for astrocytoma (CT-2A) and astrocyte (AC) cell lines were resolved on a 10% SDS polyacrylamide gel (A). The gel lanes were cut into nine gel slices followed by trypsin digestion. The peptides were analyzed by reverse-phase liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). A doubly charged [M+2H]2+ precursor ion (m/z = 618.0) from the AC membrane sample is shown in a MS survey scan (B, inset). This ion was selected for further sequence analysis by collision induced dissociation (CID) in the mass spectrometer. The MS/MS fragment ions (b- and y-ions) were later matched to the peptide sequence shown (VPVDGPPIDIGR) after searching against the mouse NCBI protein database using Mascot search algorithm. This sequence corresponded to amino acids 1707–1718 from the insulin-like growth factor-2 receptor (Supplemental Table 1).