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. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Cancer Lett. 2008 Feb 20;263(2):243–252. doi: 10.1016/j.canlet.2008.01.007

Figure 3.

Figure 3

Detection and quantification of gene expression by RT-PCR analysis of chondroitin sulfate proteoglycan-4 (Cspg4), also known as NG2 in human, and interferon-induced transmembrane protein-1, -2, and -3 (IFITM1, IFITM2, and IFITM3) (A). The gene products were detected for both AC and CT-2A cells. PCR was performed using β-actin primers as internal control and conditions as described in experimental methods. (B) The ratio of RT-PCR gene expression for each protein was compared to the β-actin internal control. The ratios of gene/β-actin are expressed as means (± 95% confidence interval) and three independent samples were analyzed for each cell line. The transcription level of each gene was compared between AC and CT-2A and statistical analysis was performed using two-tailed Student’s t-test (*p<0.01).