Figure 7.

Expression of and behavioral responses to sulfated steroids. A, ESI/MS/MS precursors of sulfate ions (m/z 97) in female mouse urine of the BALB/c strain. Inset shows corresponding result for male mouse urine, normalized to the size of the m/z 429 peak in female urine. B, The relative changes in SS425, SS427, and SS429 after restraint stress compared with unstressed controls are shown in two separate replicates. SS425 and SS427 increased by a consistent threefold after restraint stress. Pooling across all compounds, the elevation in SS levels after restraint stress is statistically significant (p < 0.01, rank-sum test).