Table 3.
Per 107 cells per hour | Per 107 nuclei per hour | ||||
Cell type | Mass, mg | Protein, μg | Nuclei, × 10-5 | Mass, mg | Protein, μg |
Wild-type | 0.83 ± 0.06 | 24 ± 1 | 9.9 ± 0.6 | 0.64 ± 0.05 | 19 ± 1 |
gα2- | 0.77 ± 0.02 | 24 ± 1 | 9.6 ± 0.4 | 0.57 ± 0.03 | 18 ± 1 |
gα5- | 0.99 ± 0.06 | 21 ± 1 | 12.6 ± 0.8 | 0.64 ± 0.05 | 13 ± 1 |
gα7- | 0.72 ± 0.06 | 23 ± 2 | 11.5 ± 1.1 | 0.51 ± 0.05 | 16 ± 2 |
gα8- | 1.43 ± 0.04** | 37 ± 2** | 15.3 ± 0.8** | 0.86 ± 0.05 | 22 ± 2 |
gα9- | 1.61 ± 0.15** | 42 ± 2** | 12.2 ± 0.5 | 1.30 ± 0.13** | 33 ± 2** |
gβ- | 1.42 ± 0.13** | 42 ± 4** | 16.2 ± 1.0** | 0.79 ± 0.08 | 23 ± 3 |
The mass, protein, and nuclei number values shown in Table 2 were divided by the observed high density doubling times from Table 1 (the mass, protein, and nuclei numbers were measured in this density range) to obtain the approximate increases in mass, protein, and nuclei per hour. All values are means ± SEM's from three or more independent assays. A ** indicates that the value was significantly different from the wild-type value with p < 0.01 (1-way ANOVA, Dunnett's test).