Table 3.

The effect of G proteins on the mass and protein increase of cells

	Per 107 cells per hour			Per 107 nuclei per hour
Cell type	Mass, mg	Protein, μg	Nuclei, × 10-5	Mass, mg	Protein, μg
Wild-type	0.83 ± 0.06	24 ± 1	9.9 ± 0.6	0.64 ± 0.05	19 ± 1
gα2-	0.77 ± 0.02	24 ± 1	9.6 ± 0.4	0.57 ± 0.03	18 ± 1
gα5-	0.99 ± 0.06	21 ± 1	12.6 ± 0.8	0.64 ± 0.05	13 ± 1
gα7-	0.72 ± 0.06	23 ± 2	11.5 ± 1.1	0.51 ± 0.05	16 ± 2
gα8-	1.43 ± 0.04**	37 ± 2**	15.3 ± 0.8**	0.86 ± 0.05	22 ± 2
gα9-	1.61 ± 0.15**	42 ± 2**	12.2 ± 0.5	1.30 ± 0.13**	33 ± 2**
gβ-	1.42 ± 0.13**	42 ± 4**	16.2 ± 1.0**	0.79 ± 0.08	23 ± 3

The mass, protein, and nuclei number values shown in Table 2 were divided by the observed high density doubling times from Table 1 (the mass, protein, and nuclei numbers were measured in this density range) to obtain the approximate increases in mass, protein, and nuclei per hour. All values are means ± SEM's from three or more independent assays. A ** indicates that the value was significantly different from the wild-type value with p < 0.01 (1-way ANOVA, Dunnett's test).