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. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Lab Chip. 2008 May 28;8(8):1386–1393. doi: 10.1039/b717043b

Fig. 3.

Fig. 3

Immunolabeling and flow cytometric analysis of adipose-derived cell mixture. (a) Ungated cytogram of starting SVF cell mixture showing the relative abundance of cellular debris, erythrocytes and leukocytes/nucleated cells. (b–d) Fluorescence (FL1) of immunolabeled cell samples was analyzed to determine the percentage of (b) NG2-positive cells (1.9%), (c) nestin-positive cells (3.9%) and (d) CD45-positive cells (3.9%) relativeto total cell number (gated to exclude submicron debris). Isotype controls were performed (data not shown).