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Flow cytometric cell count for leukocytes (CD45+, solid line) and erythrocytes (CD45-, dashed line) as a function of elution time during DEP-FFF enrichment of leukocytes from blood. More than 95% of leukocytes eluted between 15 and 17.5 min. The leukocyte/ erythrocyte ratio increased from 1:700 to 1:19, a 35-fold enrichment. The DEP-FFF was operated under a 10 kHz DEP field with the injection and withdrawal syringe pumps operated at 0.5 and 0.4 mL/ min, respectively.
