Figure 1. HSPCs from C3aR^-/- mice show defective engraftment.

This is demonstrated by a delayed recovery of platelets and leukocytes. Panel A. Lethally irradiated WT mice were transplanted with SKL cells (10⁴/mouse) isolated from C3aR-/- and WT animals. Recovery of platelet and leukocyte counts was evaluated at days 0, 5, 7, 11, 16, and 28. Panel B. Lethally irradiated C3aR^-/- mice were transplanted with SKL cells isolated from C3aR-/- and WT animals. Recovery of platelet and leukocyte counts was evaluated at days 0, 5, 7, 11, 16, and 28. The data shown represent the combined results from three independent experiments performed with 10 mice/group. *p<0.0001. All mice in irradiation control (non-transplanted) died before day 14 (not shown).