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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Leukemia. 2009 Apr 9;23(8):1455–1461. doi: 10.1038/leu.2009.73

Figure 4. C3a-C3aR axis enhances expression of MMP-9 by HSPC.

Figure 4

Sca-1+ BM cells from C3aR-/- or WT mice were non-stimulated or stimulated overnight by C3a (1 μg/ml) or desArgC3a (1 μg/ml) and mRNA was isolated for reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of MMP-9 and MMP-2 expression (Panel A). Supernatants were collected for MMP-9 analysis by zymography (Panel B). The data shown represent the combined results from three independent experiments. *p<0.0001 for real-time quantitative (RQ)-PCR. One representative zymography study is shown.

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