Figure 5. Blocking of C3aR on human UCB CD34⁺ cells impairs homing in NOD/SCID mice model.

Human UCB CD34⁺ cells were exposed or not exposed to C3aR antagonist SB 290157 and transplanted into lethally irradiated NOD/SCID mice. At 24 hours later, BM from femora of transplanted mice was isolated and the contents of human CFU-GM and femurs were evaluated in secondary in vitro cultures. The data shown represent the combined results from three independent experiments performed with 6 mice/group each. *p<0.001. No colonies grew from lethally irradiated and control animals not transplanted with UCB cells (n=5).