Table 1. The C3a-C3aR axis enhances adhesion of BM-derived Sca-1+ cells to BM stroma.
The number of CFU-GM from Sca-1+ cells from WT mice that adhered to WT strom a stimulated for 30 m in by C3a was arbitrarily assumed to be 100% in 1 hour and 3 hour adhesion assays. Data are pooled from quadruplicate samples from three independent experiments (n=12).
Source of Sca-1+ cells [adhesion time] |
Non-stimulated | Stimulated by C3a (1μg/ml, 30 min) |
Stimulated by desArgC3a (1μg/ml, 30 min) |
---|---|---|---|
WT [1 hr] | 58+/-11* | 100+/-21 | 67+/-15* |
C3aR-/- [1 hr] | 62+/-19* | 64+/-17* | 62+/-17* |
WT [3 hrs] | 49+/-11* | 100+/-8 | 46+/-13* |
C3aR-/- [3 hrs] | 53+/-16* | 49+/-20* | 51+/-7* |
p<0.00001