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Cation screening of GST-C2A-mediated membrane aggregation. Liposomes (0.5 mg/ml PC:PS) were incubated (15 min) with various divalent and monovalent cations (1 mM, all chloride salts) with or without 2.5 μM GST-C2A. The plate-reader spectrophotometer was then used to measure absorbance.