Fig. 4.

Membrane cross-linking is a conserved property of calcium-sensitive synaptogmins. (a) Top; Table indicating the degree of sequence identity between the C2AB domains of rat synaptotagmins 1–4 (Syt) and Drosophila synaptotagmin 1 (Droso1). Ability to bind phospholipid membranes in the presence of calcium is indicated. Bottom; Coomassie brilliant blue-stained polyacrylamide/SDS gel showing 3 μg of purified synaptotagmin isoforms. Molecular mass markers are indicated at the left. (b) A graph showing turbidity changes of liposomal solutions in the presence of indicated C2ABs and 1 mM calcium. Note, all tested calcium-sensitive synaptotagmin C2ABs were able to aggregate liposomes.