Figure 3.
Binding Affinities between Histone Peptides and PHD or PHD-HD1
(A) ITC profiles for the binding of methylated and unmodified 15-mer histone H3 tail peptides to free hPHD, hPHD-HD1, or dPHD-HD1 complex, as indicated in the panels; data were fitted to a one-site model. Kd values are given in the individual panels (with fitting errors indicated; see the Supplemental Experimental Procedures).
(B) Binding constants (Kd values in μM) of free human and Drosophila PHD finger versus PHD-HD1 complex for various histone ligands, as indicated.