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Intersubunit salt-bridge mutants E132Q (orange line) and R128Y (purple line) inactivate in 100 mM external K (as well as in 1 mM K), as evidenced by their failure to recover whole-cell conductance during 30 min of realkalization. Internal pH was controlled with permeant acetate buffers, and whole-cell conductances were normalized to their maximal values.
