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E132Q was inactivated in 100 mM K (green line) by oocyte acidification from pHi 8 to pHi 6.3 using permeant acetate buffers. TPNQ (500 nM) protected E132Q from inactivation in 100 mM external K (magenta line), but not from inactivation in 1 mM external K (brown line). External K was maintained constant at either 1 mM or 100 mM throughout the protocol, and whole-cell conductances were normalized to their initial values.
