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. 2009 Jul 29;106(32):13463–13468. doi: 10.1073/pnas.0906988106

Fig. 3.

Fig. 3.

STAT5 is directly involved in GATA3 and T1ST2 upregulation. (A and B) 2xTh2 cells from Stat5“floxed” or C57BL/6 mice were retrovirally infected with GFP-Cre. GFP-Cre positive cells were sorted and cultured in IL-4-containing-cRPMI for 6 days. Cells were then cultured in IL-2, IL-33, or IL-2 plus IL-33 for 48 h, with IL-4 in the medium, and then collected to measure T1st2 mRNA (A) or stained for GATA3 expression (B). (C) D7 1xTh2 cells were cultured in IL-2 plus IL-33 for an additional 2 days, and ChIP with anti-STAT5A, anti-STAT5B was performed. (D) D7 1xTh2 cells were cultured in IL-2, IL-33, or IL-2 plus IL-33 for 24 h and ChIP with anti-STAT5A, anti-STAT5B, or control mouse IgG was performed. The experiments were performed twice.