Expression and localization of NFAT isoforms in skeletal muscle. (A) Quantitative PCR of Soleus (Sol), EDL, and isolated fibers from FDB. Results are expressed as percentage of NFATc1 expression. (B) WB with antibodies specific for NFAT isoforms of total muscle extracts from Soleus (S), EDL (E), and FDB fibers (F). ▴, aspecific band.