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. 2009 Jul 24;106(32):13523–13528. doi: 10.1073/pnas.0902559106

Fig. 4.

Fig. 4.

Positive regulation of BglG-mediated antitermination does not require phosphorylation by HPr. Total RNA was prepared from the Δpts derivative of MA10 that has been transformed with a plasmid encoding either EI and HPr (lanes 1 and 2) or EI and HPr(H15A) (lanes 3 and 4) or EI(H189A) and HPr (lanes 5 and 6), grown with or without the β-glucoside salicin. The different RNA preparations were analyzed by the S1 nuclease protection assay, as in Fig. 3. The samples were separated on a denaturing polyacrylamide-urea gel. Abbreviations Cont., M, T, and AT are as in Fig. 3. The ratio between the terminated and antiterminated transcripts in Δpts MA10 transformed with a plasmid encoding EI and HPr(H15A), calculated as described in Fig. 2, in 2 independent experiments was 4.2 ± 1 (3.5 in this experiment, lane 3). The ratio between the 2 transcripts in the same strain transformed with a plasmid encoding wild-type EI and HPr, calculated from 3 independent experiments, was 2.4 ± 0.3 (2.6 in this experiment, lane 1).