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. 2009 Jul 24;106(32):13582–13587. doi: 10.1073/pnas.0906426106

Fig. 3.

Fig. 3.

Rhythmicity of ghrelin, PER1, and PER2 in oxyntic cells harvested at various ZTs and CTs in animals fed ad libitum or food-deprived. (A) Photomicrographs of a cross-section through the stomach wall show expression of ghrelin, PER1, and PER2, stained with diaminobenzidine (DAB), at 2 times of day—ZT6 and ZT18—in ad libitum-fed animals. (Scale bar: 20 μm.) (B) Quantification of ghrelin, PER1, and PER2 expression shows daily rhythms in these proteins. Note that peak expression of ghrelin occurs at ZT6, in antiphase with peak expression of PER1 and PER2. (C) Expression of ghrelin and PER1 at CT6 and CT18 in animals housed in DD for 48 h and then either fed ad libitum or food-deprived for 48 h. Note: Control sections are shown in Fig. S3. *, P < 0.05.