Fig. 3.

High glucose-induced methylglyoxal modification of p300 at R354 decreases association of p300 with HIF-1α. (A) HAECs were transfected with either Gal4(AD) wild-type p300 or p300 single-mutant 354(Q), followed by treatment for 5 days in LG or HG, and subsequent exposure to 18 h of hypoxia. Cell lysates were collected for IP of Gal4(p300) and IB for Gal4(p300) and methylglyoxal (MG). (B) Western blot quantitation of panel A. (C) HAECs were treated as in panel A. Lysates were collected for IP of HIF-1α and IB for Gal4(p300) and HIF-1α. (D) Western blot quantitation of panel C. Immunoprecipitation inputs (shown in red typeface) were used as loading controls. *, P < 0.05 vs. LG group. n = 3. AD, activation domain.