Abstract
We describe an immunofluorescence staining procedure for adenovirus in Formalin-fixed, paraffin-embedded tissue sections pretreated with a 0.25% trypsin solution. In trypsinized tissue sections stained with fluorescein-labeled antibody to adenovirus, viral antigen was brightly fluorescent and easily detected each time specimens were processed, and the nonspecific background fluorescence was always minimal. Viral antigen was nonfluorescent or only weakly fluorescent in tissue sections not pretreated with trypsin. Because pathology laboratories usually receive Formalin-fixed tissues for examination, this rapid and practical procedure can be routinely used to extend the diagnostic capability of conventional histopathology.
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- Chandler F. W., Callaway C. S., Adams S. R. Pancreatitis associated with an adenovirus in a rhesus monkey. Vet Pathol. 1974;11(2):165–171. doi: 10.1177/030098587401100208. [DOI] [PubMed] [Google Scholar]
- Ginsberg H. S. Adenoviruses. Am J Clin Pathol. 1972 Jun;57(6):771–776. doi: 10.1093/ajcp/57.6.771. [DOI] [PubMed] [Google Scholar]
- Huang S. N., Minassian H., More J. D. Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab Invest. 1976 Oct;35(4):383–390. [PubMed] [Google Scholar]
- Johnson K. P., Swoveland P. T., Emmons R. W. Diagnosis of rabies by immunofluorescence in trypsin-treated histologic sections. JAMA. 1980 Jul 4;244(1):41–43. [PubMed] [Google Scholar]
- McClure H. M., Chandler F. W., Hierholzer J. C. Necrotizing pancreatitis due to simian adenovirus type 31 in a rhesus monkey. Arch Pathol Lab Med. 1978 Mar;102(3):150–153. [PubMed] [Google Scholar]
- Morgan C., Rosenkranz H. S., Mednis B. Structure and development of viruses as observed in the electron microscope. V. Entry and uncoating of adenovirus. J Virol. 1969 Nov;4(5):777–796. doi: 10.1128/jvi.4.5.777-796.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips D. M., Raskas H. J. Ultrastructural changes in KB cultures infected with adenovirus type 2. Virology. 1972 Apr;48(1):156–169. doi: 10.1016/0042-6822(72)90123-7. [DOI] [PubMed] [Google Scholar]
- Pinkerton H., Carroll S. Fatal adenovirus pneumonia in infants. Correlation of histologic and electron microscopic observations. Am J Pathol. 1971 Dec;65(3):543–548. [PMC free article] [PubMed] [Google Scholar]
- Strano A. J. Light microscopy of selected viral diseases (morphology of viral inclusion bodies). Pathol Annu. 1976;11:53–75. [PubMed] [Google Scholar]