Fig. 2.
BdmGC-1s are N-glycosylated in HEK-293T cells. Cells transiently expressing FLAG·BdmGC-1 or FLAG·BdmGC-1Β were lysed in buffer containing protease inhibitors and incubated in G7 reaction buffer in the presence or absence of PNgaseF for 30 min at 37 °C. Western blot analysis using an anti-FLAG M2 antibody was performed.