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. 2009 Jul 28;106(32):13371–13376. doi: 10.1073/pnas.0812593106

Fig. 6.

Fig. 6.

BdmGC-1 and -1B exhibit different sensitivities to EH. (A) HEK-293T cells transiently expressing FLAG·BdmGC-1 or FLAG·BdmGC-1B were incubated with increasing concentrations of EH, i.e., 64 fM, 320 fM, 1.6 pM, 8 pM, 40 pM, 200 pM, and 1 nM, for 30 min at 37 °C. Total cGMP mobilized was measured as described in the Materials and Methods and results are expressed as mean ± SEM (n = 3). Cell lysates were immunoblotted with anti-FLAG antibodies to confirm similar expression levels of BdmGC-1 and -1B. (B) Relative increase of cGMP production in response to physiological (8 pM) or pharmacological (1,000 pM) concentrations of EH for BdmGC-1 and -1B.

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