Fig. 5.

Natural AhR ligands suppress intestinal carcinogenesis. Four to 5 mice were used in each group. Cecal carcinogenesis in the Apc^Min/+ (A) and Apc^Min/+·AhR^+/− (C) mice. Tumor development in mice fed a control diet (blue circles in A and yellow circles in C), 0.1% I3C-containing (green triangles) or 0.01% DIM-containing (beige diamondes) diet just after weaning of 3–4 weeks of age as noted by the arrows. Number of small intestinal polyps in Apc^Min/+ (B, blue squares) or Apc^Min/+·AhR^+/− (D, yellow squares) mice fed a control diet. Number of polyps in the small intestines of mice fed an I3C-containing (green squares) or DIM-containing (beige squares) diet. Data are presented as means ± SD. *, P < 0.01; **, P < 0.001; ***, P < 0.0001. (E) Representative profile of immunohistochemical staining with an antibody against β-catenin in the intestines from 15-week-old Apc^Min/+ mice fed a control or ligand-containing diet.