Fig. 1.

Ability of LNA/DNA chimeric co-oligomers to elicit RNase P-mediated cleavage of aac(6′)-Ib mRNA. (A) Induction of cleavage of ³²P-labeled aac(6′)-Ib mRNA by EGSC3 and LNA/DNA co-oligomers at 0.5 μM. Cleavage reactions were carried out as described in the Methods for 2 h in the presence of RNase P and the indicated co-oligomers (see Table 1). The products were analyzed by PAGE and phosphorimaging. Location of RNA molecular size standards (nucleotides) are shown to the left and the position of the reaction product, F249 is shown to the right. (B) RNase P and sequence-dependence of ³²P-labeled aac(6′)-Ib mRNA cleavage. Reactions were carried out as in A in the presence (+) or absence (-) of RNase P or the compounds indicated on top of the gel at 0.5 μM. S indicates that the co-oligomer has the sense sequence. (C) Kinetics of RNase P cleavage. Reactions were performed as in A for 0, 2, 5, 15, 30, 60, and 120 min using 0.05 μM of the indicated compound. The bands were quantified by densitometry.