Table 1.

A list of bacterial strains and cellulases or related enzymes from these microorganisms which have been improved using rational design or directed evolution (Modified from Percival Zhang et al., 2006).

Bacterial Strain	Enzyme	Property Altered	Method	Reference
	Rational Design
Acidothermus cellulolyticus	Endoglucanase	Type of products released	Site-directed mutation	52
Acidothermus cellulolyticus	Endoglucanase	Product inhibition	Site-directed mutation	51
Pectobacterium chrysanthami	Endoglucanase	Activity	Nonsense mutation	48
Pectobacterium chrysanthami	Endoglucanase	Activity	Insertional truncation	49
Thermobifida fusca	Processive Endoglucanase	Activity	Site-directed mutation	47
Thermotoga maritime	Endoglucanase	Activity	Site-directed mutation, CBD engineering	46
	Directed Evolution
Agrobacterium sp.	Mutated α-glucosidase	Activity	epPCR	61
Bacillus subtilis	Endoglucanase	Activity	DNA shuffling	56
Clostridium cellulovorans	Endoglucanase	Thermal stability	Family shuffling	55
Paenibacillus polymyxa	α-D-glucosidase	Thermal stability	epPCR	58
Paenibacillus polymxa	α-D-glucosidase	Thermal stability	epPCR + family shuffling	57
Pyrococcus furiousus	α-glycosidase	Activity	Family shuffling	60
Thermotoga neapolitana	α-D-glucosidase	Activity	epPCR	59