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Primers and size of PCR products
| Methods | Primers | Sequence | Length (bp) |
| QRT-PCR | PTCH1 | 5'-TGTGCGCTGTCTTCCTTCTG-3' | 119 |
| 5'-ACGGCACTGAGCTTGATTC-3' | |||
| β-actin | 5'-GCCATCCTGCGTCG-3' | 260 | |
| 5'-TGGGCACCGGAACCGCT-3' | |||
| BSP | 5'-GGGAGTATTTGGGTGGTATATT-3' | 351 | |
| 5'-AAAAAACTACAAAAAAACACCACCTTTC-3' | |||
| MSP | Methylation | 5'-GAGGGATCGATACGAATTC-3' | 143 |
| 5'-GAAAACGCGAAAAAACTAAA-3' | |||
| Non-methylation | 5'-GAGGGATTGATATGAATTT-3' | 143 | |
| 5'-AAAAACACAAAAAAACTAAA-3' |
QRT-PCR: Quantitative PCR.
