Figure 2.

Results of all the panning rounds for the first generation library of B6 scFv displaying phages. (A) Conditions used in the panning rounds. (B) Agglutination titre of clones tested from each round of panning. (C) Agglutination titre, Phage titre and sequence of V_L and V_H at the mutagenised positions in ten clones selected after panning. (a) Number of Red Blood Cells used in panning. (b) Number of phages used in panning. (c) Amount of B6 Fab protein (wild type) used in panning as competitor for binding to RBCs. (d) Phage titre determined as ampicillin resistant transductants. (e) Agglutination titre recorded as the maximum dilution of sample that gave visible agglutination (end point). (f) Percentage of clones in each panning round that gave agglutination.