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. 2009 May-Jun;1(3):268–280. doi: 10.4161/mabs.1.3.8231

Figure 8.

Figure 8

Solubility of wild type and mutant B6 Fab. PEG was used to increase the protein concentration of the Fab samples above the solubility limit. The precipitated Fab was removed by centrifugation and Fab remaining in the supernatant was quantitated by SDS-PAGE and densitometric scanning.