Reactivity of rIgY antibodies against recombinant LOX-1s. (A) Reactivity of rIgY antibodies against rhLOX-1 and delta NECK. rIgYs (1 µg/ml) were added to wells coated with biotinylated rhLOX-1 (black), delta Neck (white) or BSA (negative control, gray). (B) Cross-reactivity of 53 rIgY antibodies against LOX-1 from four species. The rIgYs (1 µg/ml) were added wells coated with biotinylated rhLOX-1 (black), rpLOX-1 (white), rrLOX-1 (gray) or rmLOX-1 (shaded). Of the 53 clones, eight cross-reacted with rrLOX-1 and/or rpLOX-1 and only two clones also recognized rmLOX-1.