Figure 4.

Depletion of non-viable hybridoma cells using Dead-Cert™ Nanoparticles. Hybridoma cells (9e10/18) taken from the end of plateau phase of culture were subjected to a single magnetic separation using Dead-Cert™ Nanoparticles. (A) Light micrographs of untreated (Control) and separated (Depleted) cells exposed to trypan blue to label dead cells. (B) Quantitative analyses of untreated and separated cell populations. Cells were counted in Neubauer haemocytometer chambers following exposure to trypan blue.