Figure 3.

Characterization of early follicle maturation defect in ovaries from adult (16 week Kit^Y719F/Kit^Y719F and sibling (+/+) controls. Tissue sections were stained for various markers as indicated. For Ki67, positivity of scattered cells serves as internal positive control; for TUNEL stains, positive cells were present elsewhere in the slide (not shown). Inhibin is expressed at similar levels in mutant and control follicles of comparable size and stage (a more advanced secondary follicle on the right side of the +/+ panel shows higher expression). Foxo3 localizes to the nucleus in primordial oocytes (inset) and shifts to the oocyte cytoplasm in primary follicles (+/+) but is abnormally retained within the nucleus of lagging primary/secondary follicles in Kit^Y719F/Kit^Y719F ovaries.